Overproduction of Thermus sp. Strain T2 beta-galactosidase in Escherichia coli and preparation by using tailor-made metal chelate supports.

Purification and Characterization of the Recombinant Thermus sp. Strain T2 a-Galactosidase Expressed in Escherichia coli

Thermoadaptation of -Galactosidase AgaB1 in Thermus thermophilus

The evolutionary potential of a thermostable -galactosidase, with regard to improved catalytic activity at high temperatures, was investigated by employing an in vivo selection system based on thermophilic bacteria. For this purpose, hybrid -galactosidase genes of agaA and agaB from Bacillus stearothermophilus KVE39, designated agaA1 and agaB1, were cloned into an autonomously replicating Therm...

Expression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants

Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...

The beta-d-galactosidase of Escherichia coli, strain K-12.

بررسی فعالیت بتاگالاکتوزیداز لاکتوباسیل های جدا شده از شیر و پنیر به روش های بیوشیمیایی و PCR

In some people the inadequate amount of Beta-galactosidase enzyme, in layer of villous epithelium of the small intestine, causes the disorder of lactose intolerance. This examination was done to observe the existence of Beta-galactosidase enzyme that is produced by separated Lactobacillus from samples of dairy products (milk and cheese). Materials and Methods: Pasteurized and local...